💉Protocol

Peptide Reconstitution 101

Step-by-step guide to mixing peptides. Water selection, dosing calculations, and proper technique.

📅 January 3, 2026⏱ 7 min read

reconstitutionprotocolhow-to

What You Need

• Lyophilized peptide vial

• Bacteriostatic water (BAC water) — preferred for multi-use vials

• Sterile water — acceptable for single-use

• Insulin syringe

• Alcohol swabs

1. Wipe the rubber stoppers of both vials with an alcohol swab

2. Draw the desired amount of BAC water into the syringe

3. Insert the needle into the peptide vial at an angle

4. Let the water run slowly down the side of the vial — do not inject directly onto the powder

5. Gently swirl (do not shake) until fully dissolved

6. The solution should be clear and colorless

If you add 1ml of water to a 5mg vial:

• You now have 5mg/ml (5000mcg/ml)

• 0.1ml = 500mcg

• 0.01ml = 50mcg

Adjust the water volume to simplify the math for your target dose.

Refrigerate at 2–8°C. Do not freeze a reconstituted vial. Use within 30 days.

For laboratory and research use only. Not intended for human consumption.