Injection Site Guide
Where and how to inject for research. Subcutaneous vs intramuscular, rotation, and best practices.
Subcutaneous (SubQ) vs Intramuscular (IM)
Most peptide research uses subcutaneous injection — into the fat layer just under the skin. This provides slower absorption and is generally easier. IM injection goes into muscle tissue and typically absorbs faster.
Common SubQ Sites
• Abdomen (2 inches from navel)
• Upper outer thigh
• Back of upper arm
Rotate sites to avoid tissue irritation and lipodystrophy.
Technique
1. Wipe site with alcohol swab, let dry
2. Pinch skin gently
3. Insert needle at 45–90° angle (90° for shorter needles/thicker fat layer)
4. Inject slowly
5. Remove needle, apply gentle pressure
6. Do not massage the site
Needle Selection
• SubQ: 27–31 gauge, 5/16" – 1/2" length
• IM: 23–25 gauge, 1" – 1.5" length
• Insulin syringes (1ml, 28–31g) work well for most peptide research volumes
Disclaimer
For laboratory and research use only. Not intended for human consumption.